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dc.contributor.authorMohammad, Ali-
dc.contributor.authorHena, Sufia-
dc.contributor.authorBhawani, Showkat Ahmad-
dc.identifier.issn0975-0975(Online); 0376-4710(Print)-
dc.description.abstractThe TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the presence of metal cation impurities. The proposed method is applicable to the identification and separation of L-tryptophan from drug samples such as Astymin -m (Forte), Astymin (liquid) and Alamin (Forte). The separation up to 10μg of L-tryptophan from milligram quantities (up to 0.77 mg) of other amino acids has also been realized. The quantitative estimation of L-tryptophan by spectrophotometry (λmax 570 nm) after separation from other amino acids has been done. The recovery of tryptophan is around 98% and its limit of detection on silica gel layer is 0.13 μg.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.ispartofseriesInt. Cl.8 G01N21/00en_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJC-A Vol.45A(07) [July 2006]en_US
dc.titleTLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determinationen_US
Appears in Collections: IJC-A Vol.45A(07) [July 2006]

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