Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/20019
Title: TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
Authors: Mohammad, Ali
Hena, Sufia
Bhawani, Showkat Ahmad
Issue Date: Jul-2006
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8 G01N21/00
Abstract: The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the presence of metal cation impurities. The proposed method is applicable to the identification and separation of L-tryptophan from drug samples such as Astymin -m (Forte), Astymin (liquid) and Alamin (Forte). The separation up to 10μg of L-tryptophan from milligram quantities (up to 0.77 mg) of other amino acids has also been realized. The quantitative estimation of L-tryptophan by spectrophotometry (λmax 570 nm) after separation from other amino acids has been done. The recovery of tryptophan is around 98% and its limit of detection on silica gel layer is 0.13 μg.
Page(s): 1663-1666
URI: http://hdl.handle.net/123456789/20019
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections: IJC-A Vol.45A(07) [July 2006]

Files in This Item:
File Description SizeFormat 
IJCA 45A(7) 1663-1666.pdf951.17 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.