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Title: In Vitro Regeneration and Hydroxycitric Acid Production in Tissue Cultures of Garcinia indica Chois
Authors: Kulkarni, Manik D
Deodhar, Manjushri A
Keywords: Garcinia indica;Organogenesis;Micropropagation;Hydroxycitric acid
Issue Date: Jul-2002
Publisher: NISCAIR-CSIR, India
Abstract: Garcinia indica is a rich source of hydroxycitric acid (HCA), an important biological active plant metabolite used as an anti-obesity drug. The female plants are the major source of main isomer, (-) - hydroxycitric acid, a powerful inhibitor of citrate cleavage enzyme. G. indica being a polygamodioecious sp, differentiation between male and female plants is detected only at the flowering stage (approx. 7 yrs). Tissue culture methods, offer scope for large scale propagation of female plants. An efficient protocol for multiple shoots and callus has been standardized to produce HCA. Multiple shoots were obtained from immature seed explants on MS basal medium supplemented with NAA (2.69 μM), BAP (8.9 μM) and KN (0.93 μM). Elongation of shoots was achieved on half MS medium supplemented with NAA (0.54 μM), BAP (0.44 μM) and KN (0.93 μM). The shoots developed roots when they were treated with 4900 μM IBA for 30 seconds and cultured on half MS basal medium. Multiple shoots and callus were obtained from explants derived from in vitro developed plantlets. The regenerated plantlets were successfully transferred to soil in pots. Multiple shoots and the callus cultures produced HCA.
Page(s): 301-304
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.01(3) [July 2002]

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