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Title: Micropropagation of Elite Cultivars of Rose-scented Geranium (Pelargonium graveolens L' Herit.) for Industrial Production of Propagules
Authors: Gupta, Ritika
Gupta, Shiv Kumar
Banerjee, S
Mallavarapu, G R
Kumar, Sushil
Keywords: Adventitious shoot proliferation;Plantlet multiplication;Geranium essential oil;In vitro propagation;Geranium planting material
Issue Date: Jul-2002
Publisher: NISCAIR-CSIR, India
Abstract: Experiments were carried out to develop an efficient micropropagation procedure to produce rooted plantlets from stem and leaf explants of the elite cultivars of rose-scented geranium, Pelargonium graveolens. A set of 8 Murashige and Skoog (MS) salts based media were evaluated for eliciting adventitious shoot regeneration response from stem and leaf explants of three cultivars namely 'Hemanti', 'Bipuli' and 'Kunti'. Stem and leaf explants without callus intervention produced 20 or more shoots in about 4 weeks. The directly regenerated shoot mass upon separation underwent good level of rooting on half strength MS medium. Plantlets thus obtained were planted in the field along with their vegetatively propagated control plants. In vitro raised plants were morphologically identical to control. Genetic fidelity of regenerants was further confirmed by gas chromatography of essential oil from aerial parts. Thus, an efficient procedure has become available for the multiplication of elite plant material of P. graveolens required for the intensive cropping in semi-temperate to tropical agro-climates of Indian hills and plains.
Page(s): 286-291
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.01(3) [July 2002]

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