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|Title:||Ethanol-induced Enhancement of the Transformation of Escherichia coli by Plasmid DNA|
|Keywords:||Ethanol;E. coli;Calcium chloride;Plasmid DNA;LPS;Transformation efficiency|
|Abstract:||The transformation efficiency (TR)E of CaCl2-treated competent E. coli cells by plasmid DNA decreased gradually by the presence of increasing ethanol concentration (up to 10% v/v) in the cell population during transformation. This was due to ethanol-induced leaching of lipopolysaccharide (LPS) from the competent cell surface. On the other hand, when the ethanol was removed after treatment of the competent cells for 30 min and then the cells were allowed to transfoml, the (TR)E enhanced compared to that of control cells; the enhancement was found to be maximum (> 100%) by the treatment of the cells with 5% (v/v) ethanol.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:|| IJBT Vol.01(2) [April 2002]|
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