Please use this identifier to cite or link to this item:
|Title:||Micropropagation of Salvadora persica Linn. via Cotyledonary Nodes|
Shekhawat, Gyan Singh
|Keywords:||Agro-forestry;Cotyledonary node;Micropropagation;Miswak;Salvadora persica|
|Abstract:||An efficient and reliable protocol for micropropagation of Salvadora persica Linn. has been standardized, which is a medicinally as well as economically important arid zone plant species. Cotyledonary nodes (1cm long) excised from 15-20 days-old seedlings germinated in vitro served as explant source. The seeds were germinated on half strength MS medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium supplemented with different concentrations of cytokinins (BAP and KN) and auxins (IAA, IBA and NAA). Maximum shoot proliferation from single explant was obtained on MS medium incorporated with BAP (4.0 mg/l), IAA (0.5 mg/l), adenine sulphate (40 mg/l), glutamine (l00 mg/l) and thiamine HCl (10 mg/l). In vitro produced shoots were induced to root on a range of IBA concentrations (0.5-5.0 mg/l) supplemented to half strength MS medium. The highest frequency of root proliferation was on half strength MS medium supplemented with 3.0 mg/l IBA. The regenerates were transferred to field conditions after acclimatization with a success rate of 60%.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:|| IJBT Vol.01(2) [April 2002]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.