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Title: Micropropagation of Salvadora persica Linn. via Cotyledonary Nodes
Authors: Mathur, Sujata
Shekhawat, Gyan Singh
Batra, Amla
Keywords: Agro-forestry;Cotyledonary node;Micropropagation;Miswak;Salvadora persica
Issue Date: Apr-2002
Publisher: NISCAIR-CSIR, India
Abstract: An efficient and reliable protocol for micropropagation of Salvadora persica Linn. has been standardized, which is a medicinally as well as economically important arid zone plant species. Cotyledonary nodes (1cm long) excised from 15-20 days-old seedlings germinated in vitro served as explant source. The seeds were germinated on half strength MS medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium supplemented with different concentrations of cytokinins (BAP and KN) and auxins (IAA, IBA and NAA). Maximum shoot proliferation from single explant was obtained on MS medium incorporated with BAP (4.0 mg/l), IAA (0.5 mg/l), adenine sulphate (40 mg/l), glutamine (l00 mg/l) and thiamine HCl (10 mg/l). In vitro produced shoots were induced to root on a range of IBA concentrations (0.5-5.0 mg/l) supplemented to half strength MS medium. The highest frequency of root proliferation was on half strength MS medium supplemented with 3.0 mg/l IBA. The regenerates were transferred to field conditions after acclimatization with a success rate of 60%.
Page(s): 197-200
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.01(2) [April 2002]

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