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Title: Determination of urinary urate with alkylamine glass-bound uricase
Authors: Pundir, C S
Bhargava, A K
Gupta, R
Issue Date: Apr-2000
Publisher: NISCAIR-CSIR, India
Abstract: Porcine liver uricase immobilized on alkylamine glass (pore diameter 55 nm) through glutaraldehyde coupling has been found useful in the determination of uric acid in urine. The minimum detection limit was 12.0 μg/0.1 ml sample and the recovery of added uric acid was 91%. The coefficient of variation (CV) were <6.0% and <2.0% for within and between batch assay respectively. A good correlation (r=0.93) was found between urate values obtained by a standard commercial method and the present method.
Page(s): 140-142
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections: IJBB Vol.37(2) [April 2000]

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