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Title: Entrapment of Sorghum root oxalate oxidase into polyvinyl alcohol membrane
Authors: Singh, Geeta
Tanwar, Durgesh N
Pundir, C S
Issue Date: Dec-2002
Publisher: NISCAIR-CSIR, India
Abstract: A Cl- and NO3- insensitive oxalate oxidase, purified from the roots of 10-day old seedlings of grain Sorghum has been immobilized on polyvinyl alcohol (PVA) membrane through entrapment with 96.07% retention of initial activity. The membrane bound enzyme showed an increase in optimum pH (from 5.0 to 6.5), time of incubation (from 5 to 10 min) and Km for oxalate (from 0.38 to 6.23 mM), but decrease in incubation temperature for maximum activity (from 37 to 30°C) and Vmax (from 70 nmol/min/ml to 9.7 nmol H2O2/min) and was unaffected by Cl- and NO3-. The membrane bound enzyme lost 50% of its initial activity after 30 days of storage at room temperature. The use of membrane bound oxalate oxidase in determination of serum oxalate of urinary stone patients is demonstrated.
Page(s): 397-400
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections: IJBB Vol.39(6) [December 2002]

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