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Title: DNA cleavage activity and cytotoxicity of mononuclear and trinuclear Cu(II) complexes containing 1H-pyrazole-3,5-dicarboxylic acid as ligand
Authors: Srivastava, Ashish Kumar
Kumari, Niraj
Khan, Rais Ahmad
Rai, Richa
Rai, Geeta
Tabassum, Sartaj
Mishra, Lallan
Keywords: Coordination chemistry;Bioinorganic chemistry;Cytotoxic activity;DNA cleavage;DNA binding;Chemical nuclease activity;Copper
Issue Date: 12-Jul-2013
Abstract: UV-vis titrations of complexes [Cu(H2pdc)2(H2O)2] (1) and [Cu3(pdc)2(bpy)2(H2O)4] (2) (H3pdc=1H-pyrazole- 3,5-dicarboxylic acid, bpy=2,2' bipyridine) with calf thymus DNA demonstrate their moderate binding with DNA. Fluorescence titrations show significant displacement of ethidium bromide bound to DNA by the complexes. Agarose gel electrophoretic mobility assay indicates that the complexes are able to cause concentration-dependent nicking of supercoiled DNA under physiological conditions. Ligation experiments with T4 DNA ligase enzyme show that the nicked DNA cleavage products generated by complexes (1) and (2) is religated to a large extent in the presence of T4 ligase enzyme. Complex (2) shows potential cytotoxicity against CaSki cervical cancer, HL-60 leukemic cell lines and THP-1 human monocytic cell lines with IC50 value of 1.5 µM, 2.5 µM and 10 µM respectively. A redox inactive Zn(II) complex of type [Zn(H2pdc)2(H2O)2]· H2O (3) was also synthesized and characterized but owing to its poor solubility, further studies could not be carried out.
Page(s): 835-844
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.52A(07) [July 2013]

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