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Title: Evaluation of Angiotensin converting enzyme inhibition and anti-oxidant activity of Piper longum L.
Authors: Chaudhary, Sushil Kumar
Mukherjee, Pulok K
Maiti, Niladri
De, Apurba Kumar
Bhadra, Santanu
Saha, Bishnu Pada
Keywords: ACE inhibitor;Piperine;Piper longum;Antioxidant;HPLC
Issue Date: Jul-2013
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, C07K 7/14, C09K 15/00, C07, C12N 9/00, C12N 11/00, A61K 38/43
Abstract: The present study was aimed to investigate the Angiotensin Converting Enzyme (ACE) inhibition and antioxidant activity of the standardized hydro alcoholic extract of Piper longum L. fruits, its fractions and isolated piperine. Extract and its fractions were standardized with reference to marker piperine by HPLC method. ACE inhibition activity was estimated using hippuryl-L-histidyl-L-leucine (HHL) as substrate. Antioxidant activity was determined by DPPH radical scavenging method. Among all the fractions tested, ethyl acetate and butanol fractions showed the maximum ACE inhibitory activity in a concentration-dependent manner with IC50 value of 1.40 ± 0.07 mg/ml and 1.75 ± 0.43 mg/ml respectively. Potential DPPH radical scavenging effect was elicited by the extract and ethyl acetate fraction with IC50 value of 193.12 ± 0.43 µg/ml and 247.78 ± 0.20 µg/ml, respectively. Though piperine showed ACE inhibition and scavenging of DPPH radical but not significant compared to the ethyl acetate fraction. P. longum can be explored further as a functional food to be useful as antihypertensive. The present experiment justifies its traditional claim as antihypertensive agent.
Page(s): 478-482
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.12(3) [July 2013]

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