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Title: Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ
Authors: Mukhopadhyay, Rupanjan
Bishayi, Biswadev
Keywords: Steroidogenic enzyme;Soluble antigen;Lymphoid organ;Corticosterone;Cytokine
Issue Date: Aug-2008
Publisher: CSIR
Abstract: The effect of soluble antigenic (bovine serum albumin, BSA) stimulation to induce steroidogenesis in murine lymphoid organs with concomitant changes in proinflammatory or inflammatory cytokine levels and its implication in the alteration of T-cell response was studied in the mice. Male Swiss albino mice (6-8 weeks old) with average body weight (20 ± 4 g) were randomly assigned to 3 groups and injected with BSA in presence and absence of Freund’s complete or incomplete adjuvant, whereas the control group received only saline. After 3 weeks, animals were sacrificed, and serums as well as lymphoid organs were collected. From the lymphoid tissue homogenate, the activities of steroidogenic enzymes and corticosterone and cytokine levels of the serum were estimated. Steroidogenic enzyme activities in murine lymphoid organs, as well as the pro-inflammatory and inflammatory cytokines levels in serum increased after Freund’s complete adjuvantemulsified BSA administration, as compared to control. The serum corticosterone and serum cytokine profile were also elevated. Results suggested that soluble protein antigen (BSA) administration stimulated steroidogenesis in murine lymphoid tissues and rise in the pro-inflammatory or inflammatory cytokine levels might indicate monocyte recruitment as well as TH₁ activation.
Page(s): 250-255
ISSN: 0301-1208
Appears in Collections: IJBB Vol.45(4) [August 2008]

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