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Title: Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis
Authors: Gao, Z Q
Meng, C X
Wang, G C
Ye, N H
Keywords: Porphyra yezoensis;Photosystem II;Filamentous sporophytes;Thylakoid membranes;Polypeptide composition;Sucrose density gradient ultracentrifugation
Issue Date: Aug-2008
Publisher: CSIR
Abstract: Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the phtosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein, D1, D2, cyt b559 and 12 kDa protein were identified from PSII particles from sporophytes; a new 102 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.
Page(s): 244-249
ISSN: 0301-1208
Appears in Collections: IJBB Vol.45(4) [August 2008]

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