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Title: | Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae (Lepidoptera) larvae |
Authors: | Xue, Chao-Bin Luo, Wan-Chun Chen, Qing-Xi Ma, De-Ying Wang, Qin |
Keywords: | Phenoloxidase;Inhibition kinetics;2-Hydroxybenzaldehyde;Monophenolase;Diphenolase;Pieris rapae (L.) |
Issue Date: | Jun-2008 |
Publisher: | CSIR |
Abstract: | Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into O-diphenols and oxidation of O-diphenols into O-quinones. In the present study, the kinetic assay for PO from Pieris rapae (L.) larvae was performed in air-saturated solutions and its kinetic behavior in the oxidation of L-tyrosine (a monophenol) and L-DOPA (L-3, 4-dihydroxyphenylalanine, a diphenol) was studied. The inhibitory effects of 2-hydroxybenzaldehyde (2-HBD) on the monophenolase and diphenolase activities of PO were also studied. Results showed that 2-HBD inhibit both the monophenolase and diphenolase activities of PO. The lag period of L-tyrosine oxidation catalyzed by the enzyme was lengthened and the steady-state activity of the enzyme sharply decreased. The inhibitor was found to be noncompetitively reversible with a KI (KI = KIS) of 1.21 mmol/L and an estimated IC₅₀ of 8.08 ± 0.11 mmol/L for monophenolase and 4.14 ± 0.08 mmol/L for diphenolase activities. In the time-course of oxidation of L-DOPA catalyzed by the enzyme in the presence of different concentrations of 2-HBD, the rate decreased with increasing time until a straight line was reached. The microscopic rate constants for the reaction of 2-HBD with the enzyme were determined. |
Page(s): | 184-191 |
URI: | http://hdl.handle.net/123456789/1931 |
ISSN: | 0301-1208 |
Appears in Collections: | IJBB Vol.45(3) [June 2008] |
Files in This Item:
File | Description | Size | Format | |
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IJBB 45(3) 184-191.pdf | 632.54 kB | Adobe PDF | View/Open |
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