|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(3) [June 2008] >
| Title: | Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1⁺ gene expression in Schizosaccharomyces pombe |
| Authors: | Chi, Zhengming
Li, Jing
Wang, Lin
He, Susu
Wang, Xianghong |
| Keywords: | MAL1⁺ gene expression
Maltase activity
Schizosaccharomyces pombe
Phosphatidylinositol
Glucose |
| Issue Date: | Jun-2008 |
| Publisher: | CSIR |
| Abstract: | In our previous study, we found that the transformant Sch.p944 which contains the plasmid pADH-INO synthesized
higher inositol than the transformant Sch.p1025 which contains the plasmid pSPIN-22. In this study, we examined the
influence of INO1 gene expression on intracellular maltase activity and MAL1⁺ gene expression in these two transformants.
The highest specific maltase activity was observed when Sch.p944 was grown in the synthetic medium containing initial
2.0% (w/v) glucose, whereas glucose repression of maltase activity occurred when Sch.p1025 was cultivated in the same
medium containing glucose more than initial 0.5% (w/v), suggesting that synthesis of the maltase was derepressed in
Sch.p944. Also, higher mRNA encoding intracellular maltase activity was found in Sch.p944 than that in Sch.p1025 after 32
h of cell growth in the media containing initial 2.0% glucose, indicating that the derepression occurred at the transcriptional
level. Furthermore, higher phosphatidylinositol (PI) content was detected in Sch.p944 cells grown in the medium containing
initial 2.0% (w/v) glucose for 32 h than in Sch.p1025 cells grown under the same conditions, indicating that PI might be
involved in derepression of MAL1⁺ gene expression and intracellular maltase activity. |
| Page(s): | 174-178 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.45(3) [June 2008]
|
|
