Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/1928
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dc.contributor.authorKoti, Jaya S-
dc.contributor.authorKanugula, Sreenivas-
dc.contributor.authorSuryanarayana, Tangirala-
dc.date.accessioned2008-08-29T11:10:15Z-
dc.date.available2008-08-29T11:10:15Z-
dc.date.issued2008-06-
dc.identifier.issn0301-1208-
dc.identifier.urihttp://hdl.handle.net/123456789/1928-
dc.description166-173en_US
dc.description.abstractThe solution structure of an archaeal DNA binding protein Sac10b (DBNP-B) by cross-linking with formaldehyde and its interaction with DNA were studied. Results indicated that Sac10b existed as oligomeric structure in solution and the oligomerization was greatly stimulated by Mg²⁺ ions and elevated temperatures. In light of our earlier observation that Sac10b interacts with DNA forming different types of complexes with DNA at different protein concentrations, its DNAbinding properties were also studied. Results demonstrated that the protein formed rapidly sedimentable co-aggregation complexes with both native and denatured DNA in a protein concentration-dependent manner. These protein DNA complexes were fluid-like crystalline material at a protein DNA ratio (3-6:1 w/w). Gel mobility shift assays carried out to study the interaction of the protein with plasmid DNA indicated possible DNA nicking by the protein. The DNA nicking activity of Sac10b was optimal in pH range of 7-8.5 and was dependent on Mg²⁺ ions. It was maximal at protein to DNA ratio of (8:1, w/w) and very little activity was observed above and below this ratio. Nicking of DNA at this ratio indicated structure-specific DNA nicking by the protein. The protein might have important multi-functional role in the DNA metabolism in this organism.en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.sourceIJBB Vol.45(3) [June 2008]en_US
dc.subjectSulfolobus acidocaldariusen_US
dc.subjectSac10ben_US
dc.subjectDNA aggregationen_US
dc.subjectDNA nickingen_US
dc.titleDNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activityen_US
dc.typeArticleen_US
Appears in Collections: IJBB Vol.45(3) [June 2008]

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