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Title: A new marker IS1311 L2 PCR-REA for identification of ‘Indian Bison’ type Mycobacterium avium subspecies paratuberculosis
Authors: Sohal, J S
Singh, S V
Singh, P K
Singh, AV
Kumar, Naveen
Keywords: Diagnosis;Indian Bison type S5;Molecular marker;Mycobacterium avium subspecies paratuberculosis
Issue Date: Apr-2013
Publisher: NISCAIR-CSIR, India
Abstract: Previously, a new biotype (Indian Bison type S5) of Mycobacterium avium subspecies paratuberculosis (MAP), genetically different from so far reported MAP genotypes, was identified. This biotype is widely distributed in different geographical regions in India among various host species including humans. Due to deletion of TG at 64th and 65th of IS1311 element at locus 2 (L2), there is a loss of BsaJI restriction site in Indian Bison type MAP S5. However, in non-Indian MAP (cattle type/sheep type/US Bison type), the site was intact. Taking advantages of these variations in the genome, authors optimized a new marker IS1311 L2 PCR-REA (Restriction Endonuclease Assay), capable of distinguishing Indian Bison type MAP S5 from other biotypes. To test the field applicability, newly optimized assay was applied on 66 DNA samples (27 from cultural isolates and 39 from clinical specimens) where it successfully distinguished the MAP of Indian Bison type from other MAPs. Hence, this assay can be used to characterize MAP isolates in future molecular epidemiology investigations. Also the applicability of this assay on clinical DNA samples gives us freedom to have knowledge of the infecting MAP genotype prior to culture.
Page(s): 204-207
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(2) [April 2013]

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