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Title: First report on IS elements of Shigella flexneri 1a— A common Indian isolate
Authors: Ranade, Suvidya
Khandekar, P S
Chopade, B A
Deobagkar, D N
Keywords: ISEhe3;IS elements;S. flexneri 1a;Sequencing;Shotgun cloning
Issue Date: Apr-2013
Publisher: NISCAIR-CSIR, India
Abstract: Shigella species are non-sporulating, Gram negative, facultative anaerobes. IS (insertion sequence) elements are the major cause of the dynamics of Sf301 chromosome and due to IS-mediated DNA rearrangements and formation of pseudogenes, the Shigella spp. became highly specific human pathogens with variable epidemiological and pathological features. Nucleotide sequence analysis of S. flexneri 1a genomic DNA was performed through Big dye terminator chemistry using ABI 3730 48 capillary DNA analyzer. In total, 60 kb data in form of contigs were analyzed by homology search using various bioinformatics tools. IS elements were identified using nucleotide blast at NCBI as well as the Is finder. Eight different IS elements were identified, which were present in different copy number. A new IS element ISEhe3 was identified, which belonged to family IS3. ISEhe3 was found absent in S. flexneri 2a 301, but it was present in 2457T strain. Amongst the IS elements identified, IS2, IS3 and IS600 elements showed identity with SHI2 Shigella pathogenicity island. The presence of large numbers of IS-elements in the Shigella genomes is likely the major cause of many of the genome rearrangements. Further investigations on IS elements will help to study genome dynamics and rearrangement of S. flexneri 1a strain.
Page(s): 199-203
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(2) [April 2013]

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