Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/19180
Title: Sperm-mediated gene transfer into oocytes of the golden hamster: Assessment of sperm function
Authors: Fernandeza, M A
Mani, S A
Rangarajan, P N
Seshagiri, P B
Issue Date: Nov-1999
Publisher: NISCAIR-CSIR, India
Abstract: The possibility of sperm as a vehicle to deliver foreign DNA to oocytes was tested in hamsters. Epididymal spermatozoa, incubated with linearized plasmid DNA encoding ovine growth hormone (pCMXoGH), showed a spontaneous tendency to interact with DNA. Kinetics of sperm uptake of DNA was determined by using [32P]-labeled DNA. Spermatozoa took up the added DNA by 15-30 min and the uptake was inhibited by human seminal fluid in a dose dependent manner. Addition of DNA did not affect the functional competence of spermatozoa, in terms of their ability to undergo capacitation and acrosome reaction (34.5%±2.2 vs 35%±1.5). The fertilizing ability of DNA treated-spermatozoa from hamsters and humans was assessed by zona-free hamster egg penetration assay. Number of sperm penetrated peroocyte were 23±4.5 and 1.4±1.3 for hamster and human spermatozoa, respectively. Penetrated oocytes harbored sperm-treated DNA both with hamster (30.2 cpm/oocyte) and human ( 19.2 cpm/oocyte) spermatozoa. These results show that the hamster and human spermatozoa have a strong tendency to interact with exogenous (foreign) DNA and are able to transfer DNA to oocytes. Sperm may be used as a vector for DNA transfer and this approach has potential in the production of transgenic animals.
Page(s): 1085-1092
URI: http://hdl.handle.net/123456789/19180
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(11) [November 1999]

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