Association of an unknown potyvirus isolate with a severe mosaic disease of <i>Narcissus tazetta </i>L.

Abstract

An isolate of potyvirus was found to be associated with severe mosaic disease in <i>Narcissus tazetta </i>L. Electron microscopy, SDS-polyacrylamide gel electrophoresis of coat protein, serological relationship and RT-PCR were carried out to identify the virus isolate. Electron microscopy of purified virus preparation revealed the presence of flexuous particles of 730 ×13 nm. One band with a molecular weight of 33 kDa was obtained by SDS-polyacryl amide gel electrophoresis. Serological relationship of the virus isolate with bean yellow mosaic virus was established in electroblot immunoassay. Employing potyvirus specific primers in RT-PCR, a band of about 300 bp was obtained from the core region o f the coat protein, thus confirming the association of a potyvirus with the mosaic disease of <i>N. tazetta.</i>