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Title: Association of an unknown potyvirus isolate with a severe mosaic disease of Narcissus tazetta L.
Authors: Aminuddin
Khan, J A
Raj, S K
Issue Date: Oct-1999
Publisher: NISCAIR-CSIR, India
Abstract: An isolate of potyvirus was found to be associated with severe mosaic disease in Narcissus tazetta L. Electron microscopy, SDS-polyacrylamide gel electrophoresis of coat protein, serological relationship and RT-PCR were carried out to identify the virus isolate. Electron microscopy of purified virus preparation revealed the presence of flexuous particles of 730 ×13 nm. One band with a molecular weight of 33 kDa was obtained by SDS-polyacryl amide gel electrophoresis. Serological relationship of the virus isolate with bean yellow mosaic virus was established in electroblot immunoassay. Employing potyvirus specific primers in RT-PCR, a band of about 300 bp was obtained from the core region o f the coat protein, thus confirming the association of a potyvirus with the mosaic disease of N. tazetta.
Page(s): 1034-1036
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(10) [October 1999]

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