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Title: Detection, prevalence, purification and characterization of lecithinase of Klebsiella pnemoniae
Authors: Singh, B R
Sharma, V D
Chandra, R
Issue Date: Sep-1999
Publisher: NISCAIR-CSIR, India
Abstract: Lecithinase activity in Klebsiella is a rare trait as out of 208 strains of Klebsiella belonging to 3 species, viz. K. pnemoniae (168), K. planticola (29) and K. oxytoca (II), only 4 strains of K. pneumoniae produced lecithinase positive colonies on egg-yolk-agar. Although cell lysates of 16 K. pnemoniae yielded positive results for lecithinase assay on egg-yolk agar,19 strains were detected positive for lecithinase with ELISA using anti-lecithinase serum. Release of up to 52.12 % cell-bound lecithinase could be achieved with polymyxin-B treatment at 100 μg/ml concentration. Purified lecithinase was determined to be a high molecular weight (70 kDa), crystalizable, anionic (pI, 3.5) protein. It possessed cytolytic, haemolytic and dermonecrotic activities but did not induce fluid accumulation in rabbit ileal loop or infant mouse guts. It was inactivated by boiling, trypsin and chymotrypsin treatment and alkaline pH. Serologically, it was related to lecithinase of Aeromonas caviae and phospholipase-C of Salmonella.
Page(s): 925-932
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections: IJEB Vol.37(09) [September 1999]

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