Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/19098
Title: Monoclonal antibodies against bovine growth hormone
Authors: Kumar, Pradeep
Rajput, Yudhishthir S.
Issue Date: Jul-1999
Publisher: NISCAIR-CSIR, India
Abstract: A number of mouse x mouse hybridomas producing monoclonal antibodies (MAbs) against bovine growth hormone (bGH) were prepared by fusion of spleen cells from bGH-primed mice (Balb/c) with non-secretory mouse myeloma cells (PAIOP3) and characterized. MAbs obtained from three fusion experiments belonged to IgM, IgG1 and IgG2b class/subclass of antibodies. Cross-reaction studies indicated that generated antibodies were against three different epitopes of bGH. VIA6E8 (IgG1) and VIIB2E11C9 (IgM) did not cross-react with ovine prolactin (oPRL), ovine leutinizing hormone (oLH) and porcine follicle stimulating hormone. Antibody VIB3C9E8 (IgM) exhibited cross-reaction with oPRL and oLH. Antibody VIC 1 F9 (IgG2b) cross reacted with oPRL. All MAbs were against conformational epitopes of bGH.
Page(s): 650-654
URI: http://hdl.handle.net/123456789/19098
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(07) [July 1999]

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