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Title: In vitro regeneration of cashew (Anacardium occidentale L.)*
Authors: Thimmappaiah
Samuel, Shirly Raichal
Issue Date: Apr-1999
Publisher: NISCAIR-CSIR, India
Abstract: Axillary shoot bud proliferation (1-13 shoot buds per explant) was observed when shoot-tip/ nodal explants from in vitro grown seed lings of cashew were cultured on three fourth strength Murashige and Skoog medium supplemented with thidiazuron (0.05 to 2 mg L-1) alone and or in combination with other plant growth regulators (BAP, NAA, IBA).Medium containing thidiazuron (0.1 mg L-1) in combination with NAA or IBA (0.1 mg L-1) showed maximum proliferation (>4.0 buds/explant). Roots were induced in vitro when individual shoots were initially cultured on half strength MS semi-solid medium with auxins and subsequently transferred to hormone free half-strength MS medium. Medium containing NAA (5mg L-1) or AA plus IBA (2.5 mg L-1 each) combination induced maximum rooting (50%). Liquid medium gave higher percentage of rooting than agar medium. WPM liquid medium containing 2.5 mg L-1 each of NAA and IBA was found best for rooting in vitro with 80% response. Ex vitro rooting was also observed but to a limited extent (12.5%). Rooted plantlets were hardened both in lab and green house and 29 of them have been field planted for further evaluation.
Page(s): 384-390
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(04) [April 1999]

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