Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/19023
Title: Stress-mediated regeneration from mature embryo-derived calli and gene transfer through Agrobacterium in rice (Oryza sativa)
Authors: Gandhi, Rita
Khurana, Paramjit
Issue Date: Apr-1999
Publisher: NISCAIR-CSIR, India
Abstract: Mature seed embryos of five different genotypes of indica rice namely, IR72, IR36, rasi, mahsuri and jaya produced calli (100%) on MS medium supplemented with 2,4-D (10μM). A high frequency of plantlet formation (100% for rasi) was obtained when calli were regenerated on hormonal (10 μM  , Kn and 5 μM, NAA) medium solidified with 1.6% of agar instead of 0.8% which is normally employed. This simple water stress treatment was effective for plantlet regeneration even in the absence of hormones. The stress treatment was also effective in other indica genotypes of rice which normally regenerate poorly on a basal or a hormone-supplemented media, and promoted shoot regeneration from long term callus cultures of rice variety rasi. Use or'mature seed embryos for Agrobacterium-mediated transformation resulted in GUS expression in scutellum and shoot region of 4-days old germinated embryos.
Page(s): 332-339
URI: http://hdl.handle.net/123456789/19023
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(04) [April 1999]

Files in This Item:
File Description SizeFormat 
IJEB 37(4) 332-339.pdf1.82 MBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.