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|Title:||Influence of ammonium to nitrate nitrogen ratio in different basal media on callusing response of capsicum (Capsicum annuum L. grossum Sendt.) anthers*|
|Authors:||Mythili, J B|
|Abstract:||Capsicum (cv. arka gaurav) anthers were cultured on MS, B5, NN and RO media containing sucrose (3%). kinetin (0.5-5.0μM) and 2,4-D (0.5 or 20.0μM) or IAA (5.0μM). callusing response of anthers averaged over different hormonal treatments and incubating environments was significantly higher in 135 (45.5%) followed by RO (40.1%), NN (34.7%) and MS (29.8%). The response was found to be inversely related to NH4+/NO3- ratio (0.08, 0.22, 0.49 and 0.52 respectively) in the medium but not sensitive to total N concentration. Reducing NH4+/NO3- ratio in MS or NN to the level of B5 without altering total N content significantly increased the per cent callusing in them. Conversely, increasing proportion of NH4+ in RO medium inhibited callusing. Complete replacement of NH4+ with NO3- as N source severely inhibited callusing indicating that a reduced form of N was essential. Callusing response was insensitive to a wide range of N concentration in the basal medium as long as NH4+/NO3- ratio was maintained.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.37(03) [March 1999]|
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