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Title: Production of esculin by hairy root cultures of chicory (Cichorium intybus Lucknow local)
Authors: George, Jacob
Bais, Harshpal
Ravishankar, G A
Issue Date: Mar-1999
Publisher: NISCAIR-CSIR, India
Abstract: Hairy root cultures established with Agrobacterium rhizogenes LMG 150 of an Indian cultivar of chicory produced esculin, a dihydroxycoumarin monoglucoside used as skin protectant and a marker in microbiological media. Maximum biomass of 6.2±0.425 g/culture of transformed roots was obtained in full strength MS medium with 3% sucrose on 28th day. Esculin production was linked to growth and 100 g FW of transformed roots gave a maximum of 13.93 mg, corresponding to a ~13-fold increase as compared to that in 30-day-old non-transformed roots (0.72 mg), during the same period.
Page(s): 269-273
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(03) [March 1999]

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