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|Title:||Production of esculin by hairy root cultures of chicory (Cichorium intybus L.cv. Lucknow local)|
Ravishankar, G A
|Abstract:||Hairy root cultures established with Agrobacterium rhizogenes LMG 150 of an Indian cultivar of chicory produced esculin, a dihydroxycoumarin monoglucoside used as skin protectant and a marker in microbiological media. Maximum biomass of 6.2±0.425 g/culture of transformed roots was obtained in full strength MS medium with 3% sucrose on 28th day. Esculin production was linked to growth and 100 g FW of transformed roots gave a maximum of 13.93 mg, corresponding to a ~13-fold increase as compared to that in 30-day-old non-transformed roots (0.72 mg), during the same period.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.37(03) [March 1999]|
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