Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/18979
Title: Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture
Authors: Prasad, G
Minakshi
Issue Date: Feb-1999
Publisher: NISCAIR-CSIR, India
Abstract: Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 106 TCID50/ml . RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on to-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA- PAGE and DIA was 105 TCID50/ml.
Page(s): 157-160
URI: http://hdl.handle.net/123456789/18979
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(02) [February 1999]

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