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|Title:||Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture|
|Abstract:||Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 106 TCID50/ml . RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on to-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA- PAGE and DIA was 105 TCID50/ml.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.37(02) [February 1999]|
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