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|Title:||Gallotannin hydrolysis by immobilized fungal mycelia in a packed bed bioreactor|
|Abstract:||Hydrolysis of gallotannin to gallic acid by immobilized mycelia of Aspergillus niger MTCC 282, Aspergillus fischerii MTCC 150, Fusarium solani MTCC 350 and Trichoderma viride MTCC 167 in a packed bed bioreactor was studied. Fungal mycelia pre induced with 5 g L-1 gallotannin were immobilized in calcium alginate gel (1.5%) and the resultant beads were packed in a column to a bed volume of 175 mm3. Gallotannin dissolved in distilled water was passed through the column and the eluate was recycled after adjusting pH to 6 with ammonium hydroxide (10%). Maximwn hydrolysis of gallotannin was recorded by immobilized mycelia of F. solani and T. viride at 35° and 45°C after 175 and 60 min of residency period respectively. Optimum substrate concentration required for maximum hydrolysis was 10g L-1 at pH 5 for both the fungi. Immobilized mycelia of A. niger and A. fischerii revealed maximum operational stability. Loss of activity after eighth run was in the order of - A. niger (no loss), A. fischerii (7.5%), F. solani (18%) and T viride (18%). Stability in terms of retention of enzyme activity after 150 days of storage at 4°C was A. niger (58%), A. fischerii (26.8%), F. solani (83%) and T viride (85 .1 %).|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.37(01) [January 1999]|
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