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Title: Comparative study of catalytic activity of immobilized invertase in sodium alginate gel on sucrose hydrolysis
Authors: Khobragade, C N
Chandel, S G
Issue Date: Nov-2002
Publisher: NISCAIR-CSIR, India
Abstract: Invertase is an intracellular hydrolytic enzyme widely used in the inversion of sucrose into glucose and fructose. Both glucose and fructose are the primary compounds used as sweeteners in the food processing industries. In the present investigation invertase was immobilized in sodium alginate gel by entrapment method and the catalytic activity of immobilized enzyme was compared with the activity of native enzyme at variable pH and temperatures. Experimental findings revealed that for native enzyme optimum activity was at pH 4.2 and at temperature 30°C, while for immobilized enzyme it was observed at pH 4.8 and at temperature 50°C. Kinetic parameters Km and Vmax as well as catalytic efficiency of invertase was also determined for both the native and immobilized enzyme.
Page(s): 535-539
ISSN: 0975-0991 (Online); 0971-457X (Print)
Appears in Collections:IJCT Vol.09(6) [November 2002]

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