Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/18692
Title: Important role of the C-terminal region of pig aldo-keto reductase family 1 member C1 in the NADPH-dependent reduction of steroid hormones
Authors: Kwon, Seul Gi
Bang, Woo Young
Jeong, Jin Young
Cho, Hwak Rae
Park, Da Hye
Hwang, Jung Hye
Kim, Tae Wan
Ko, Moon-Suck
Cho, In-Cheol
Joo, Young Kuk
Jeong, Mi-Ae
Kim, Sam Woong
Kim, Chul Wook
Keywords: AKR1C1;NADPH-dependent reduction;Steroid hormone
Issue Date: Jun-2013
Publisher: NISCAIR-CSIR, India
Abstract: The NADPH-dependent reduction activities of two paralogous pig AKR1C1s with and without 19 additional amino acid residues in C-terminus were evaluated against steroid hormones including 5a-dihydrotestosterone, testosterone, progesterone, androstenedione and 5a-androstane-3,17-dione, which act as substrates of the AKR1C1s. Among the hormones, the AKR1C1s exhibited the highest activity against 5a-dihydrotestosterone and the lowest activity against testosterone and progesterone. Furthermore, the AKR1C1s showed the largest differential activities against 5a-dihydrotestosterone, but no such change of activities was found against progestrone and testosterone. These results suggest that the C-terminal region of AKR1C1 plays an important effect in the reduction activities of pig AKR1C1. Thus, the differential activities of two AKR1C1 paralogs observed in the present study provide important insights in understanding the molecular evolution.
Page(s): 237-241
URI: http://hdl.handle.net/123456789/18692
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.50(3) [June 2013]

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