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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >
| Title: | Role of calcium ions and temperature on dextransucrase production |
| Authors: | Qader, Shah Ali Ul
Aman, Afsheen
Bano, Saeeda
Syed, Noman
Azhar, Abid |
| Keywords: | Dextran
CaCl₂
Stability
Dextransucrase
Glucosyl-transferase
Leuconostoc mesenteroides |
| Issue Date: | Jul-2008 |
| Publisher: | CSIR |
| Abstract: | Dextransucrase [E.C. 2.4.1.5] enzyme from newly isolated strain of Leuconostoc mesenteroides PCSIR-4 showed maximum production in the fermentation medium containing 0.005% CaCl₂. The production of dextransucrase was 2.5 times higher in the medium containing calcium ions as compared to the medium without calcium ions. Also, the stability of the enzyme increased up to120 d. Maximum dextransucrase production was obtained when culture was incubated at 25C, while extracellular enzyme activity at 35C. Thermal stability of extracellular enzyme was maximum at 30C and at 50C the total enzyme loss was in
240 min. |
| Page(s): | 404-406 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(3) [July 2008]
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