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Title: Role of calcium ions and temperature on dextransucrase production
Authors: Qader, Shah Ali Ul
Aman, Afsheen
Bano, Saeeda
Syed, Noman
Azhar, Abid
Keywords: Dextran
CaCl₂
Stability
Dextransucrase
Glucosyl-transferase
Leuconostoc mesenteroides
Issue Date: Jul-2008
Publisher: CSIR
Abstract: Dextransucrase [E.C. 2.4.1.5] enzyme from newly isolated strain of Leuconostoc mesenteroides PCSIR-4 showed maximum production in the fermentation medium containing 0.005% CaCl₂. The production of dextransucrase was 2.5 times higher in the medium containing calcium ions as compared to the medium without calcium ions. Also, the stability of the enzyme increased up to120 d. Maximum dextransucrase production was obtained when culture was incubated at 25C, while extracellular enzyme activity at 35C. Thermal stability of extracellular enzyme was maximum at 30C and at 50C the total enzyme loss was in 240 min.
Page(s): 404-406
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

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