Please use this identifier to cite or link to this item:
http://nopr.niscair.res.in/handle/123456789/18591
Title: | Simple and sensitive determination of nucleic acid by Rayleigh light scattering technique with methyl green-CTMAB |
Authors: | Liu, Rutao Yang, Jinghe Wu, Xia Sun, Changxia |
Issue Date: | Oct-2001 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | A method is presented for the determination of nucleic acid, based on the enhancement of Rayleigh light scattering (RLS) of methyl green (MG)-CTMAB in the pH range 6.9-7.2 at 414.0 nm. The enhanced intensity of RLS is proportional to the concentration of nucleic acid in the range 2.5×10-8-2.0×10-6g/ml for calf thymus (ct)DNA, 2.5×10-8-2.0×10-6g/ml for fish sperm (fs)DNA, 7.5×10-8-2.0×10-6g/ml for yeast (y)RNA. The detection limits (3σ) are 7.8, 2.6, 9.9 ng/ml for ctDNA, fsDNA and yRNA respectively. Besides its high sensitivity, it has some other advantages: simplicity of operation, commonality of spectrometer and reagents, good stability of chemical system and reproducibility. The procedure has been successfully applied to the determination of nucleic acid in synthetic samples. |
Page(s): | 1121-1123 |
URI: | http://hdl.handle.net/123456789/18591 |
ISSN: | 0975-0975(Online); 0376-4710(Print) |
Appears in Collections: | IJC-A Vol.40A(10) [October 2001] |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
IJCA 40A(10) 1121-1123.pdf | 691.4 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.