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Title: Simple and sensitive determination of nucleic acid by Rayleigh light scattering technique with methyl green-CTMAB
Authors: Liu, Rutao
Yang, Jinghe
Wu, Xia
Sun, Changxia
Issue Date: Oct-2001
Publisher: NISCAIR-CSIR, India
Abstract: A method is presented for the determination of nucleic acid, based on the enhancement of Rayleigh light scattering (RLS) of methyl green (MG)-CTMAB in the pH range 6.9-7.2 at 414.0 nm. The enhanced intensity of RLS is proportional to the concentration of nucleic acid in the range 2.5×10-8-2.0×10-6g/ml for calf thymus (ct)DNA, 2.5×10-8-2.0×10-6g/ml  for fish sperm (fs)DNA, 7.5×10-8-2.0×10-6g/ml for yeast (y)RNA. The detection limits (3σ) are 7.8, 2.6, 9.9 ng/ml for ctDNA, fsDNA and yRNA respectively. Besides its high sensitivity, it has some other advantages: simplicity of operation, commonality of spectrometer and reagents, good stability of chemical system and reproducibility. The procedure has been successfully applied to the determination of nucleic acid in synthetic samples.
Page(s): 1121-1123
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.40A(10) [October 2001]

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