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Title: In vitro shoot proliferation in Emblica officinalis var. Balwant from nodal explants
Authors: Goyal, Divya
Bhadauria, Seema
Keywords: Emblica officinalis
Micropropagation
Nodal shoots
Amla
Issue Date: Jul-2008
Publisher: CSIR
Abstract: An efficient protocol for in vitro shoot proliferation of Emblica officinalis var. Balwant has been developed by using nodal explants. In vitro shoot proliferation from nodal explants have been observed by using MS, B₅ and WPM media. Amongst them, MS medium has been observed the best for shoot proliferation (63.3 %), while minimum phenol leaching (63.3 %) was observed on WPM medium. To enhance the shoot regenerative potential, explants were cultured on MS medium supplemented with various cytokinins, viz., BAP, Kn and TDZ. MS medium supplemented with BAP (4.44 μM /L) was observed the best among all cytokinins. As shoot proliferation depended upon the balance of cytokinins and auxins, and BAP was found ideal for shoot proliferation, additive effect of auxins – IAA, IBA and NAA was assessed at 4.44 μM /L conc. of BAP. Explants when cultured on MS + BAP (4.44 μM/L) with IBA (2.46 μM /L)) showed maximum percentage of shoots (85 %) with minimum callus formation (15%). Higher concentration of auxins suppressed the caulogenesis and induced callus formation.
Page(s): 394-397
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

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