NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >

Title: In vitro propagation and quercetin quantification in callus cultures of Rasna (Pluchea lanceolata Oliver & Hiern.)
Authors: Arya, Deepika
Patni, Vidya
Kant, Uma
Keywords: In vitro propagation
Pluchea lanceolata
Issue Date: Jul-2008
Publisher: CSIR
Abstract: A protocol for micropropagation of Pluchea lanceolata, an important medicinal herb was developed. Leaf explants obtained from field grown plants when tested for callus induction on Murashige and Skoog’s (MS) medium, supplemented with NAA in combination with BAP, produced the best callus. Maximum number of multiple shoots from the callus (26.6±0.67) was obtained on MS medium supplemented with BAP (1.0 mg/L) and Kn (1.0 mg/L). More or less uniform elongation of multiple shoots was obtained on MS medium with lower concentrations of cytokinins, i.e., BAP (0.25 mg/L) and Kn (0.5 mg/L). Further elongation and profuse rooting were achieved when the well-grown shoots were cultured on half strength MS medium supplemented with IBA (1.0 mg/L). The regenerated plantlets were hardened and established at 70% survival rate in pots. The bioactive secondary metabolite, quercetin, was isolated from callus tissues of different age groups and its identification and confirmation was carried out by the colour reaction, TLC behaviour, IR spectrum and HPLC techniques. Maximum quercetin content (0.23 mg/g dry wt of tissue) was obtained in 6-wk-old callus tissues.
Page(s): 383-387
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

Files in This Item:

File Description SizeFormat
IJBT 7(3) 383-387.pdf315.87 kBAdobe PDFView/Open
 Current Page Visits: 328 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 163259 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: