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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >
| Title: | Production of alkaline protease by Bacillus circulans using agricultural residues: A statistical approach |
| Authors: | Jaswal, R K
Kocher, G S
Virk, M S |
| Keywords: | Alkaline protease
Bacillus circulans
CN combination
Cotton deoiled mea
Regression analysis l
Soybean meal |
| Issue Date: | Jul-2008 |
| Publisher: | CSIR |
| Abstract: | Bacillus circulans, an alkaline protease producer isolated from vegetable waste, showed a pH of 10.5, temperature between 25-30°C and agitation rate of 200 rpm as optimum physical conditions for enzyme production. Factorial experiments were designed and the best CN combination of glucose and soybean meal produced a maximum alkaline protease of 461.65 U/mL. Soybean meal replaced with cotton deoiled meal (CDM) from enzyme production medium as the maximum supporter of alkaline protease produced 589 U/mL of protease. Protease values with different concentrations of CDM fitted in a regression equation showed that B. circulans produces maximum alkaline protease of 808.68 U mL⁻¹ in 100.9 h using 0.5789% CDM. |
| Page(s): | 356-360 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(3) [July 2008]
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