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Title: Production of alkaline protease by Bacillus circulans using agricultural residues: A statistical approach
Authors: Jaswal, R K
Kocher, G S
Virk, M S
Keywords: Alkaline protease
Bacillus circulans
CN combination
Cotton deoiled mea
Regression analysis l
Soybean meal
Issue Date: Jul-2008
Publisher: CSIR
Abstract: Bacillus circulans, an alkaline protease producer isolated from vegetable waste, showed a pH of 10.5, temperature between 25-30°C and agitation rate of 200 rpm as optimum physical conditions for enzyme production. Factorial experiments were designed and the best CN combination of glucose and soybean meal produced a maximum alkaline protease of 461.65 U/mL. Soybean meal replaced with cotton deoiled meal (CDM) from enzyme production medium as the maximum supporter of alkaline protease produced 589 U/mL of protease. Protease values with different concentrations of CDM fitted in a regression equation showed that B. circulans produces maximum alkaline protease of 808.68 U mL⁻¹ in 100.9 h using 0.5789% CDM.
Page(s): 356-360
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

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