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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >
| Title: | Enhanced production of streptomycin and hydrolytic enzymes by Streptomyces griseus strains using different types of organic solvents and detergent compounds |
| Authors: | El-shahed, Kamal Y I
El-Diwany, Ahmed I
Awad, Hassan M M |
| Keywords: | Streptomyces griseus
Streptomycin
Amylases
Proteases
Organic solvents
Detergent compounds |
| Issue Date: | Jul-2008 |
| Publisher: | CSIR |
| Abstract: | Different organic solvents and detergents showed profound effects on the production of streptomycin (SM), amylases and proteases by Streptomyces griseus strains. The maximum SM production (1128 and 780 mg/L) was obtained using Streptomyces NRRL-strain when ethanol (1.0% v/v) and Tween-80 (0.1%v/v) were added to the medium. Production of amylases was also enhanced using the strain with the addition of benzene at decline phase, n-butanol at the trophophase and isopropanol at the idiophase with values, 10.66, 8.28 and 8.05 U/mL for -amylase (EC-3.2.1.1) and 6.28, 4.68 and 4.55 for -amylase (EC-3.2.1.2), respectively. While the use of other solvents and detergents reduced the production of amylase specially activities by S. griseus DSM-40759, characterized by higher productions of - and -amylases in different growth phases especially at the death phase. Worthily, proteases secretion was only induced to 1.02 U/mL at the idiophase by culturing DSM-strain with the addition of Triton X-100 and SDS to the fermentation medium. Moreover, the solvents shifted the pH values in the acidic range by the two strains at different phases compared to the control. |
| Page(s): | 341-348 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(3) [July 2008]
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