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Title: Enhanced production of streptomycin and hydrolytic enzymes by Streptomyces griseus strains using different types of organic solvents and detergent compounds
Authors: El-shahed, Kamal Y I
El-Diwany, Ahmed I
Awad, Hassan M M
Keywords: Streptomyces griseus
Streptomycin
Amylases
Proteases
Organic solvents
Detergent compounds
Issue Date: Jul-2008
Publisher: CSIR
Abstract: Different organic solvents and detergents showed profound effects on the production of streptomycin (SM), amylases and proteases by Streptomyces griseus strains. The maximum SM production (1128 and 780 mg/L) was obtained using Streptomyces NRRL-strain when ethanol (1.0% v/v) and Tween-80 (0.1%v/v) were added to the medium. Production of amylases was also enhanced using the strain with the addition of benzene at decline phase, n-butanol at the trophophase and isopropanol at the idiophase with values, 10.66, 8.28 and 8.05 U/mL for -amylase (EC-3.2.1.1) and 6.28, 4.68 and 4.55 for -amylase (EC-3.2.1.2), respectively. While the use of other solvents and detergents reduced the production of amylase specially activities by S. griseus DSM-40759, characterized by higher productions of - and -amylases in different growth phases especially at the death phase. Worthily, proteases secretion was only induced to 1.02 U/mL at the idiophase by culturing DSM-strain with the addition of Triton X-100 and SDS to the fermentation medium. Moreover, the solvents shifted the pH values in the acidic range by the two strains at different phases compared to the control.
Page(s): 341-348
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

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