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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >
| Title: | Immobilization of porcine pancreas lipase onto free and affixed arylamine glass beads and its application in removal of oil stains |
| Authors: | Sharma, M
Kumar, V
Pundir, C S |
| Keywords: | Porcine pancreas lipase
Arylamine glass beads
Immobilization
Detergent
Cloth washing
Oil stains |
| Issue Date: | Jul-2008 |
| Publisher: | CSIR |
| Abstract: | Porcine pancreas lipase has been immobilized through diazotization onto free and affixed arylamine glass beads with 75.59% and 54.26% retention of the initial activity of free enzyme and conjugation yield of 16 mg g⁻¹ and 7 mg g⁻¹, respectively. Optimum pH of the enzyme was decreased on free glass beads but increased on affixed glass beads. Optimum temperature, energy of activation (Ea), time of incubation and Km for triolein were increased but Vmax remained almost unchanged after immobilization on both free and affixed arylamine glass beads. The utility of immobilized enzyme in the removal of oil stains from cotton cloth by various detergents was tested by chemical method. All the detergents gave better wash performance in the presence of immobilized lipase (both onto free as well affixed glass beads) than that by detergent alone. Furthermore, the washing by cheaper (non-enzymic) detergents in the presence of immobilized lipase was almost similar to that by expensive (enzymic) detergents. The free and affixed bead-bound enzyme could be used repeatedly about 100 times without any considerable loss of activity. |
| Page(s): | 328-332 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(3) [July 2008]
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