NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >


Title: RAPD based assessment of genetic diversity of Butea monosperma from different agro-ecological regions of India
Authors: Vaishali
Khan, Suphiya
Sharma, Vinay
Keywords: DNA
Butea monosperma
molecular profiling
RAPD
Agro- ecological regions
Issue Date: Jul-2008
Publisher: CSIR
Abstract: Butea monosperma (Lam.) Taub. (English. Flame of the forest) belonging to the family Fabaceae, is an anthropogenic tree of several castes and also a very useful tree for both local people and pharmaceutical industry. This valuable tree needs attention for the characterization of its genetic diversity, protection and cultivation. The present work describes randomly amplified polymorphic DNA (RAPD) analysis to assess the genetic divergence among 16 Butea accessions collected from four agro-ecological regions of India (nine agro-climatic sub zones), coverring five states (Uttarakhand, Uttar Pradesh, Rajasthan, Madhya Pradesh and Karnataka). Out of the 30 ten mer random primers used for studying genetic divergence, 12 were polymorphic, generating a total of 145 amplification products with an average of 12 products per polymorphic primer and an estimated mean gene diversity of 0.43. Genetic relationships among accessions were evaluated by generating a similarity matrix based on Jaccard’s coefficient ranging from 0.53 to 0.79. The phenetic dendrogram generated by UPGMA analysis grouped accessions into four clusters. Primer 5, 12 and 16 were found most informative based on their resolving power and their potential to differentiate all the accessions. The degree of genetic variation detected among the 16 accessions with RAPD analysis suggested that RAPD could be used for studying genetic diversity in Butea. The study also demonstrated that Butea germplasm collected from different agro-ecological regions showed no isolation based on sub-climatic zones as the accessions collected from different sub-climatic zones grouped together in the genetic tree.
Page(s): 320-327
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

Files in This Item:

File Description SizeFormat
IJBT 7(3) 320-327.pdf550.98 kBAdobe PDFView/Open
 Current Page Visits: 770 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 639899 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: nopr@niscair.res.in