NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(3) [July 2008] >

Title: Assessment of genetic variation among populations of Rhynchostylis retusa, an epiphytic orchid from Goa, India using ISSR and RAPD markers
Authors: Parab, G V
Krishnan, S
Keywords: Rhynchostylis retusa
Genetic variation
Issue Date: Jul-2008
Publisher: CSIR
Abstract: Rhynchostylis retusa (L.) Bl., a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence, ranks among the important Indian ornamental orchids. Comparative population studies using PCR based markers, RAPD and ISSR, were performed to assess the genetic diversity of the wild orchid. Among the 35 primers tested, 13 RAPD and 7 ISSR primers were selected for the analysis. In total, 74 RAPD and 30 ISSR fragments were generated. High level of polymorphism was recorded in RAPD (76.13%) than ISSR (62.6%). In case of RAPD, Nei’s average genetic identities value for different populations of R. retusa ranged from 0.405 to 0.932. While for ISSR, it ranged from 0.733 to 0.933. The results of the present study can be seen as starting point for future research on the population and evolutionary genetics of this species.
Page(s): 313-319
ISSN: 0972-5849
Source: IJBT Vol.07(3) [July 2008]

Files in This Item:

File Description SizeFormat
IJBT 7(3) 313-319.pdf858.1 kBAdobe PDFView/Open
 Current Page Visits: 125 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 172229 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: