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|Title:||Micropropagation of a rare mangrove Bruguiera cylindrica L. towards conservation|
|Keywords:||Bruguiera cylindrica L;Conservation;Hypocotyl;in vitro propagation;Tree mangrove|
|Abstract:||An in vitro propagation protocol has been developed for Bruguiera cylindrica L. (Rhizophoraceae), a rare tree mangrove found along the western coast of India. Hypocotyl segments of viviparous propagule were cultured in vitro on Murashige and Skoog’s medium (MS). Shoot and root initiation was better on modified MS (MSM) than on MS. Shoots and roots were produced from the cut surface simultaneously in 74% explants on MSM devoid of growth regulators. The explants produced several visible shoot buds on their cut surfaces, though only 1-3 shoots were developed per explant. Multiple shoots were produced by 70% explants. When cultures were initiated in monsoon, 1.6-fold increase in shoot formation and 2.4-fold increase in root formation was observed over those initiated before monsoon. During acclimatization the survival was 87%. Considering the status of this species in terms of its distribution, threats and conservation values in the study area, its in vitro propagation will compliment and strengthen the large-scale plantation activities towards the conservation or restoration of mangrove forests.|
|Appears in Collections:|| IJBT Vol.07(2) [April 2008]|
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