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Title: Thin layer chromatographic detection of poly-β-hydroxybutyrate (PHB) and poly-β-hydroxyvalerate (PHV) in cyanobacteria
Authors: Panda, Bhabatarini
Sharma, Laxuman
Singh, Akhilesh Kumar
Mallick, Nirupama
Keywords: Cyanobacteria;Gas chromatography;β-hydroxybutyrate;β-hydroxyvalerate;Propanolysis;Thin layer chromatography
Issue Date: Jun-2008
Publisher: CSIR
Abstract: A simple method for the detection of poly-β-hydroxybutyrate (PHB) and poly-β-hydroxyvalerate (PHV) by thin layer chromatography was developed. Using this method, PHB and PHV were detected in four cyanobacterial species, viz. Nostoc muscorum, Spirulina platensis, Calothrix sp. and Synechocystis sp. PCC 6803. Presence of hydroxybutyrate (HB) units and hydroxyvalerate (HV) units was confirmed by UV-spectrophotometric and gas chromatographic analyses.
Page(s): 230-234
ISSN: 0972-5849
Appears in Collections: IJBT Vol.07(2) [April 2008]

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