Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/1832
Title: Biotechnological production of xylitol by mutant Candida tropicalis OMV5: Process optimization using statistical approach
Authors: Rao, Ravella Sreenivas
Jyothi, Cherukuri Pavana
Rao, Linga Venkateswar
Keywords: Candida tropicalis OMV5;Mutant;Orthogonal array;Statistical optimization;Taguchi methodology;Xylose;Xylitol
Issue Date: Apr-2008
Publisher: CSIR
Abstract: Received 22 January 2007; revised 17 August 2007; accepted 25 October 2007 An orthogonal experimental design L16 (25) was used to investigate effects of key media components namely xylose, yeast extract, peptone, urea and inoculum size on the production of xylitol by a mutant strain of Candida tropicalis (CT-OMV5). Software for automatic design and analysis of the experiments, based on Taguchi approach was used. Optimal levels of key media components were also determined. Among the tested parameters, xylose and urea contributed higher influence and yeast extract concentration also played an important role in the conversion of xylose to xylitol. Application of Taguchi method helped in easy process optimization and higher xylitol yield. The increased yield was possible at lower levels of yeast extract and peptone. The yield of xylitol under these optimal conditions was 0.89g/g of xylose.
Page(s): 218-224
URI: http://hdl.handle.net/123456789/1832
ISSN: 0972-5849
Appears in Collections: IJBT Vol.07(2) [April 2008]

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