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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(2) [April 2008] >
| Title: | Biotechnological production of xylitol by mutant Candida tropicalis OMV5: Process optimization using statistical approach |
| Authors: | Rao, Ravella Sreenivas
Jyothi, Cherukuri Pavana
Rao, Linga Venkateswar |
| Keywords: | Candida tropicalis OMV5
Mutant
Orthogonal array
Statistical optimization
Taguchi methodology
Xylose
Xylitol |
| Issue Date: | Apr-2008 |
| Publisher: | CSIR |
| Abstract: | Received 22 January 2007; revised 17 August 2007; accepted 25 October 2007
An orthogonal experimental design L16 (25) was used to investigate effects of key media components namely xylose, yeast extract, peptone, urea and inoculum size on the production of xylitol by a mutant strain of Candida tropicalis (CT-OMV5). Software for automatic design and analysis of the experiments, based on Taguchi approach was used. Optimal levels of key media components were also determined. Among the tested parameters, xylose and urea contributed higher influence and yeast extract concentration also played an important role in the conversion of xylose to xylitol. Application of Taguchi method helped in easy process optimization and higher xylitol yield. The increased yield was possible at lower levels of yeast extract and peptone. The yield of xylitol under these optimal conditions was 0.89g/g of xylose. |
| Page(s): | 218-224 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(2) [April 2008]
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