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Title: Molecular characterization of Tobacco streak virus causing soybean necrosis in India
Authors: Kumar, N Arun
Narasu, M Lakshmi
Zehr, Usha B
Ravi, K S
Keywords: Coat protein gene
Tobacco streak virus
Issue Date: Apr-2008
Publisher: CSIR
Abstract: A virus isolate infecting soybean (Glycine max L.) with characteristic symptoms of necrosis was collected from various locations of Maharashtra, India. The virus was detected as Tobacco streak virus (TSV) by direct antigen-coating-enzyme-linked immunosorbent assay (DAC-ELISA) using TSV specific antiserum. ELISA positive TSV soybean isolates upon mechanical transmission onto indicator hosts, viz. Vigna unguiculata cv. C-152, Glycine max and Nicotiana tabacum cv. Xanthi, produced characteristic local necrotic lesions on primary inoculated leaves, followed by systemic infection. Coat protein (CP) gene from a representative soybean isolate (TSV-SB) was amplified using TSV CP specific primers. The amplicon of ~750 bp was cloned and sequenced. The CP gene consists of 717 nucleotides, potential of coding a polypeptide of 238 amino acid residues. The CP gene of TSV-SB isolate shared 98.3 to 99.3% nucleotide and 96.6 to 98.3% amino acid sequence homology with the TSV isolates characterized from India. With TSV-WC (White clover isolate from America, type strain) and TSV-BR (Soybean isolate from Brazil), TSV-SB isolate shared 88.7% and 79.2% amino acid sequence homology, respectively. Phylogenetic tree constructed based on amino acid sequence analysis showed a close clustering of TSV-SB isolate with other TSV isolates from India than with the American and Brazilian strains. Based on CP gene sequence analysis, it is concluded that the TSV-SB isolate is a strain of TSV that is prevalent in India. This is the first report of molecular characterization of TSV infecting soybean from India.
Page(s): 214-217
ISSN: 0972-5849
Source: IJBT Vol.07(2) [April 2008]

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