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Title: Expression of gene encoding immunodominant merozoite surface protein of Theileria annulata in Escherichia coli
Authors: Rajendran, C
Ray, D D
Bansal, G C
Keywords: Theileria annulata;Immunodominant merozoites surface protein;pPROEXHTb;E.coli;Ni-NTA affinity chromatography;Western blot analysis
Issue Date: Apr-2008
Publisher: CSIR
Abstract: The asexual blood stage “merozoites” of an Indian strain of the tick-borne cattle haemoprotozoa, Theileria annulata, was generated in in vitro culture and the gene encoding the merozoite surface protein (Tams 1) was amplified from cDNA by using primers designed from T. annulata (Ankara strain). The amplified gene was cloned into pPROExHT b plasmid vector and expressed as fusion protein in Escherichia coli, DH5α strain. The recombinant protein was purified using NI-NTA affinity chromatography and used to detect antibody response in known anti-Tannulata bovine serum samples in a ELISA format.
Page(s): 200-203
ISSN: 0972-5849
Appears in Collections: IJBT Vol.07(2) [April 2008]

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