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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(2) [April 2008] >
| Title: | Enhanced accumulation of triterpenoids and flavonoids in cell suspension cultures of Azadirachta indica (A. Juss.) with an extended stationary phase |
| Authors: | Babu, Vidhu Sankar
Narasimhan, S
Nair, G M |
| Keywords: | Azadirachta indica
Azadirachtin
Kaempferol
Nimbin
Quercetin
Salannin
Stationary phase
Suspension culture |
| Issue Date: | Apr-2008 |
| Publisher: | CSIR |
| Abstract: | Accumulation of three triterpenoids (azadirachtin-A, nimbin and salannin) and two flavonoids (quercetin and kaempferol) was analyzed in cell suspension cultures of Azadirachta indica
A. Juss. By feeding cells with a sterile solution of sucrose, it was possible to sustain viability of cells in batch cultures without subculturing for 60 d. Such long-term cell suspension cultures exhibited an extended steady stationary phase which facilitated enhanced accumulation of azadirachtin-A, nimbin, salannin, quercetin and kaempferol. 40% of the cells were viable towards the end of 60 d of incubation. Maintaining cells for longer time under stationary phase facilitated transformation of cells from low productivity to high productivity. The cells in stationary phase could serve as a starting material for the selection of elite cell lines and for bioreactor cultures for large-scale production of these compounds. |
| Page(s): | 270-272 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(2) [April 2008]
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