Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/18149
Title: Determination of amino acids separated by micellar electrokinetic chromatography after pre-column derivatization with o-phthalaldehyde
Authors: Durgbanshi, A
Sharma, S
Shelke, M
Bose, D
Asthana, A
Sanghi, S K
Issue Date: May-2004
Publisher: NISCAIR-CSIR, India
IPC Code: Int.Cl.7 B01D 15/08
Abstract: A simple, sensitive and rapid analytical method has been developed for the determination of amino acids separated by micellar electrokinetic chromatography after pre-column derivatization with o-phthalaldehyde. The separated amino acids have been detected using ultraviolet as well as electrochemical detection modes. The OPA derivatized amino acids have been separated on a 75μm (internal diameter) fused silica capillary. UV detection has been performed at 220 and 335 nm respectively,while electrochemical detection has been done at 500 mV against Ag/AgCl reference electrode. Optimum separation has been achieved using an applied voltage of 28 kV. Enhanced sensitivity is achieved with the electrochemical detection mode. The standard curve of amino acids is found to be linear in the range of 10-5 to 5×10-7 M. The % relative standard deviation for retention time is found to be less than 0.4 while for peak height and peak area it is within 1%.
Page(s): 1095-1098
URI: http://hdl.handle.net/123456789/18149
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.43A(05) [May 2004]

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