|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.67 [2008] >
JSIR Vol.67(08) [August 2008] >
| Title: | Studies on calcium alginate-pectin gel entrapped concanavalin A-bitter gourd (Momordica charantia) peroxidase complex |
| Authors: | Satar, Rukhsana
Matto, Mahreen
Husain, Qayyum |
| Keywords: | AlginateBitter gourd, Concanavalin A, Entrapped, Pectin, Peroxidase
Alginate
Bitter gourd
Concanavalin A
Entrapped
Pectin
Peroxidase |
| Issue Date: | Aug-2008 |
| Publisher: | CSIR |
| Abstract: | Ammonium sulfate fractionated proteins of bitter gourd (Momordica charantia) were precipitated using jack bean
extract as a source of concanavalin A. Concanavalin A-bitter gourd peroxidase retained nearly 86% original activity. This
complex was entrapped into calcium alginate-pectin gel. Entrapped concanavalin A-peroxidase complex retained 51%
original activity. Soluble and immobilized peroxidase preparations exhibited maximum activity at 40°C and pH 5.5.
Concanavalin A-peroxidase complex and entrapped concanavalin A-enzyme complex retained greater fraction of catalytic
activity at higher temperatures as compared to soluble form and also showed more broadening in pH-activity profiles,
indicating a marked increase in stability. Concanavalin A-bitter gourd peroxidase and calcium alginate-pectin entrapped
concanavalin A-bitter gourd peroxidase preparations were more stable as compared to soluble counterpart against denaturation
induced by heat, pH, urea, organic solvents and detergents. |
| Page(s): | 609-615 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.67(08) [August 2008]
|
|
