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|Title:||Effect of crude oil concentrations, temperature and pH on growth and degradation of crude oil by marine bacteria|
|Authors:||Vyas, Trupti K.|
Dave, B. P.
Growth of bacteria
|Abstract:||Accidental spillage of petroleum in marine environment provokes serious damage to marine ecosystem. Microbial degradation of petroleum hydrocarbons is one of the major practice in the natural decontamination process. Out of 130 crude oil degrading bacteria isolated from Bhavnagar (Alang) coast, NW India, 12 isolates designated as TV 1 – TV 12 that formed maximum clearing zone on mineral medium were examined for their hydrocarbon degradation potential in temporal sequence of events. Among these, isolate TV 11 showed maximum growth (0.70 mg/ml) and degradation (22.8 %) on 34th day, followed by TV 4 that showed maximum growth (0.60 mg/ml) and degradation (17.0 %) and TV 3 that showed maximum growth (0.59 mg/ml) and degradation (16.2 %) on 28th day of incubation. Isolate TV 11 was identified as Marinococcus albus, TV 3 as Methylobacterium mesophilicum and TV 4 as Nocardia otitidiscaviarum. Effect of various crude oil concentrations (2 - 6 g/l), temperature (20 – 37 ºC) and pH (6 - 9) on growth and degradation revealed that 5 g/l of crude oil concentration, 30 ºC temperature for M. albus and 25 ºC for M. mesophilicum and N. otitidiscaviarum and pH 7 were optimum for both growth and degradation. Enhanced bacterial growth and degradation achieved with optimized concentrations of crude oil, temperature and pH can make their use suitable in devising an appropriate bioremediation strategy for oil contaminated sites.|
|Appears in Collections:||IJMS Vol.36(1) [March 2007]|
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|IJMS 36(1) (2007) 76-85.pdf||499.94 kB||Adobe PDF||View/Open|
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