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Title: Fungal Cells Turgor Pressure: Theoretical Approach and Measurement
Authors: Gervais, Patrick
Abadie, Christophe
Molin, Paul
Issue Date: Sep-1999
Publisher: NISCAIR-CSIR, India
Abstract: Hyphal growth has been examined from a mechanistic standpoint to understand the role of turgor pressure on hyphal extension rate. From a rigourous momentum balance it is shown that turgor pressure can be expressed as a sum of two components :(i) a static contribution, related to the membrane elastic properties and resolved by capillary probe measurements; and (ii) a dynamic part (ΔP), responsible for the hyphal extension rate and proportional to it. Both contributions add up and explain why some researchers have found hyphae growing at finite rates when the capillary probe measures no pressure difference at all; it is simply because the dynamic part of the turgor pressure cannot be resolved by such type of technique. The linear relationship between turgor pressure and hyphal extension rate means that two measurements need to be taken in order to completely characterize the microscopic parameters involved in this phenomenum. An experimental setup is proposed based on quick osmotic shocks upon the hyphae — to stop tip growth without inducing plasmolysis— to accomplish such a discrimination. This approach has been applied to Aspergillus orizae and an increasing relation between the dynamic part of turgor pressure  (ΔP) and hyphal extension rate has been found .
Page(s): 671-677
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.58(09) [September 1999]

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