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Title: Characterisation of sapindosides in Sapindus mukorosii saponin (reetha saponin) and quantitative determination of sapindoside B*
Authors: Saxena, D
Pal, R
Dwivedi, A K
Singh, S
Keywords: Sapindus saponian;Sapindoside-B;Spermicidal;HPLC;HPTLC;LC-MS
Issue Date: Feb-2004
Publisher: NISCAIR-CSIR, India
Abstract: High performance thin layer chromatography (HPTLC) method as well as high performance liquid chromatographic (HPLC) method combined with ES-MS are developed and validated for fingerprinting (profiling) Sapindus saponin and quantitative determination of Sapindoside B in bulk drug samples of Sapindus saponin and its formulation Consap cream. The separation of saponins using TLC is achieved on precoated silica gel plates using chloroform: methanol: water as mobile phase. Detection of the spots is done at the 630 nm. The identification of saponins is done by LC-MS, using Electro Spray Ionisation [ESI] technique. The HPLC method involves chromatography of the saponins on reverse phase (C-18) column, using acetonitrile-water gradient as mobile phase. The detection is done by UV -visible detector at a wavelength of 215 nm. Calibration graphs are found to be linear over the range 11-220 μg in HPTLC and 30-200 μg/mL in HPLC method. The methods developed are being used for the analysis of the bulk drug samples and Consap cream samples.
Page(s): 181-186
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.63(02) [February 2004]

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