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Title: New reagent for determination of trace levels of nitrite in environmental samples
Authors: Reddy, M C Somasekhara
Keywords: Nitrite determination;Spectrophotometry;4,4'-diaminostilbene-2,2' -disulphonic acid;2-Naphthol;Pink azo dye
Issue Date: Feb-2004
Publisher: NISCAIR-CSIR, India
Abstract: A local industrial product an intermediate dye disodium salt of 4,4-diaminostil bene-2,2-disulphonic acid is introduced as a new reagent for a selective and sensitive spectrophotometric determination method of trace levels of nitrite in environmental samples like, water and soil. The method is based on the tetraazo coupling reaction of disodium salt of 4,4-diaminostilbelle-2,2-disulphonic acid with a coupling agent of 2-naphthol. The stable water soluble pink azo dye formed under aqueous alkaline condition has an adsorption maximum at 520 nm. The reaction is fast. The dye formed is stable for more than 48 h. Beer's law is obeyed between 0.04-1.6 ppm of nitrite in a final volume of 25 mL. The molar extinction coefficient of the color system is 3.0×10L/mole/cm. Sandal sensitivity is 1.5335×10-3 ppm/cm2 The relative standard deviation is 2.1 per cent for 10 determinations at 10 ppm level. The method is applied to the determi nation of nitrite in water and soil samples and the results are compared with the results obtained from the standard method.
Page(s): 172-176
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.63(02) [February 2004]

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